Anticorps Polyclonal de lapin anti-USP49

• Phare
• Validé par KD/KO

USP49 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 18066-1-AP

Synonymes

Ubiquitin-specific-processing protease 49, Ubiquitin thiolesterase 49, Ubiquitin thioesterase 49, Ubiquitin carboxyl-terminal hydrolase 49, EC:3.4.19.12

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 18066-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18066-1-AP (USP49 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18066-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18066-1-AP (USP49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18066-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18066-1-AP (USP49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18066-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18066-1-AP (USP49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 16 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

18066-1-AP cible USP49 dans les applications de WB, IHC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP49 Protéine recombinante Ag12697
• Nom complet: ubiquitin specific peptidase 49
• Masse moléculaire calculée: 73 kDa, 79 kDa
• Poids moléculaire observé: 79-85 kDa
• Numéro d’acquisition GenBank: BC014176
• Symbole du gène: USP49
• Identification du gène (NCBI): 25862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USPs are cysteine proteases that vary greatly in size and structural complexity. Outside of the core catalytic domain, USPs exhibit N-terminal and C-terminal extensions that have been proposed to play roles in determining cellular localization and substrate specificity of these enzymes(PMID:14715245). USP49(Ubiquitin carboxyl-terminal hydrolase 49) belongs to this family. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis.; Authors - Kamini Kaushal ; KO Validated
human	WB	EMBO J; USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.; Authors - Kuntian Luo ; KD Validated
human	WB	Theranostics; USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2; Authors - Arun Pandian Chandrasekaran ; KD Validated
human	WB	Elife; USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication.; Authors - Ting Pan ; KD Validated
human	WB,CoIP	Carcinogenesis; USP49 promotes adenocarcinoma of the esophagogastric junction malignant progression via activating SHCBP1-β-catenin-GPX4 axis; Authors - Yun Ding ; KD Validated
human	WB	J Cell Mol Med; CCAT1/FABP5 promotes tumour progression through mediating fatty acid metabolism and stabilizing PI3K/AKT/mTOR signalling in lung adenocarcinoma.; Authors - Jing Chen

{{ptg:RelatedPrimaryAntibodies}}