Anticorps Monoclonal anti-USP5

• Phare
• Validé par KD/KO

USP5 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F12C10

N° de cat : 66213-1-PBS

Synonymes

3F12C10, Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT

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Galerie de données de validation

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  WB analysis using 66213-1-Ig (same clone as 66213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66213-1-Ig (same clone as 66213-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

  WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66213-1-Ig (same clone as 66213-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51440-1

  Cytometric bead array sample test of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS.

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  Cytometric bead array standard curve of MP51440-1

  Cytometric bead array standard curve of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS. Standard:Ag7575. Range: 1.563-200 ng/mL.

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Informations sur le produit

66213-1-PBS cible USP5 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP5 Protéine recombinante Ag7575
• Nom complet: ubiquitin specific peptidase 5 (isopeptidase T)
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 95-105 kDa
• Numéro d’acquisition GenBank: BC005139
• Symbole du gène: USP5
• Identification du gène (NCBI): 8078
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

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