Anticorps Recombinant de lapin anti-USP53

USP53 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240971A6

N° de cat : 83846-1-PBS

Synonymes

240971A6, Inactive ubiquitin carboxyl-terminal hydrolase 53, Inactive ubiquitin-specific peptidase 53, KIAA1350

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Galerie de données de validation

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  WB analysis using 83846-1-RR (same clone as 83846-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83846-1-RR (USP53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83846-1-RR (same clone as 83846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 83846-1-RR (same clone as 83846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00821-1

  Cytometric bead array standard curve of MP00821-1, USP53 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83846-1-PBS. Detection antibody: 83846-2-PBS. Standard: Ag28923. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00821-2

  Cytometric bead array standard curve of MP00821-2, USP53 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83846-1-PBS. Detection antibody: 83846-3-PBS. Standard: Ag28923. Range: 1.56-100 ng/mL.

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  Affinity and Kinetic Characterization of 83846-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83846-1-RR against Human USP53 were performed. The affinity constant is 0.878 nM.

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Informations sur le produit

83846-1-PBS cible USP53 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: USP53 Protéine recombinante Ag28923
• Nom complet: ubiquitin specific peptidase 53
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_019050
• Symbole du gène: USP53
• Identification du gène (NCBI): 54532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin specific peptidase 53 (USP53) was originally discovered in cholestasis, and its tumor suppressive effects have recently been reported in various cancers. Zhao et al. showed that USP53 interacted with FKBP51 in lung adenocarcinoma cells, and induced apoptosis via the AKT pathway. Furthermore, USP53 inhibited the progression of clear cell renal cell carcinoma by suppressing the nuclear factor κB (NF‐κB) pathway. (PMID: 35654790)

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