Anticorps Polyclonal de lapin anti-USP7

• Phare
• Validé par KD/KO

USP7 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 26948-1-AP

Synonymes

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7

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Galerie de données de validation

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  WB analysis using 26948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26948-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26948-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26948-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 26948-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 26948-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26948-1-AP (USP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26948-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26948-1-AP (USP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules U2OS, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

26948-1-AP cible USP7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP7 Protéine recombinante Ag25634
• Nom complet: ubiquitin specific peptidase 7 (herpes virus-associated)
• Poids moléculaire observé: 126-128 kDa
• Symbole du gène: USP7
• Identification du gène (NCBI): 7874
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	Cancer Res; Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.; Authors - Monika Vishnoi
human	WB	Front Immunol; Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.; Authors - Wenying Gao ; KD Validated
human	WB,IHC	Int J Biol Sci; Flap endonuclease 1 Facilitated Hepatocellular Carcinoma Progression by Enhancing USP7/MDM2-mediated P53 Inactivation.; Authors - Saiyan Bian
human	WB	Discov Oncol; High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer; Authors - Haiqing Wang
mouse	WB,IHC	Front Pharmacol; Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice; Authors - Yu-Hui Gu

Produits associés

MDM2 Polyclonal antibody

19058-1-AP