Anticorps Monoclonal anti-USP7

• Phare
• Validé par KD/KO

USP7 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D7D11

N° de cat : 66514-1-PBS

Synonymes

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7

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Galerie de données de validation

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  WB analysis using 66514-1-Ig (same clone as 66514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66514-1-Ig (same clone as 66514-1-PBS)

  WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected HeLa cells. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66514-1-Ig (same clone as 66514-1-PBS)

  WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected A549 cells. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66514-1-Ig (same clone as 66514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66514-1-Ig (same clone as 66514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66514-1-Ig (same clone as 66514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66514-1-Ig (same clone as 66514-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66514-1-Ig (USP7 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66514-1-PBS cible USP7 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP7 Protéine recombinante Ag25652
• Nom complet: ubiquitin specific peptidase 7 (herpes virus-associated)
• Poids moléculaire observé: 128 kDa
• Symbole du gène: USP7
• Identification du gène (NCBI): 7874
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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