Anticorps Monoclonal anti-USP8

• Phare
• Validé par KD/KO

USP8 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

1E12E3

N° de cat : 67321-1-Ig

Synonymes

1E12E3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055

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Galerie de données de validation

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  WB analysis using 67321-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67321-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67321-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67321-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67321-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67321-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67321-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67321-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (67321-1-Ig, Clone: 1E12E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of Hela using 67321-1-Ig

  1X10^6 Hela cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 67321-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67321-1-Ig cible USP8 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP8 Protéine recombinante Ag27104
• Nom complet: ubiquitin specific peptidase 8
• Masse moléculaire calculée: 1118 aa, 128 kDa
• Poids moléculaire observé: 128 kDa
• Numéro d’acquisition GenBank: BC110590
• Symbole du gène: USP8
• Identification du gène (NCBI): 9101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

Publications

Species	Application	Title
human	WB,IHC,IF	Cell Death Dis; USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization; Authors - Jianing Tang
human	WB	J Hum Genet; A heterozygous germline deletion within USP8 causes severe neurodevelopmental delay with multiorgan abnormalities; Authors - Masamune Sakamoto
human	WB	Cell Death Dis; Mechanical signal modulates prostate cancer immune escape by USP8-mediated ubiquitination-dependent degradation of PD-L1 and MHC-1; Authors - Zhi-Bin Ke
human	WB,IP,IF,CoIP	Adv Sci (Weinh); Targeting USP8 Inhibits O-GlcNAcylation of SLC7A11 to Promote Ferroptosis of Hepatocellular Carcinoma via Stabilization of OGT; Authors - Jianing Tang
human	WB	J Adv Res; The ubiquitin-specific protease 8 antagonizes melatonin-induced endocytic degradation of MT(1) receptor to promote lung adenocarcinoma growth; Authors - Qianhui Sun ; KD Validated

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