Anticorps Monoclonal anti-USP8

USP8 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E12E3

N° de cat : 67321-1-PBS

Synonymes

1E12E3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055

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Galerie de données de validation

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  WB analysis using 67321-1-Ig (same clone as 67321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67321-1-Ig (same clone as 67321-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67321-1-Ig (same clone as 67321-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67321-1-Ig (same clone as 67321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67321-1-Ig (same clone as 67321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (67321-1-Ig, Clone: 1E12E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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  FC experiment of Hela using 67321-1-Ig (same clone as 67321-1-PBS)

  1X10^6 Hela cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67321-1-PBS cible USP8 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP8 Protéine recombinante Ag27104
• Nom complet: ubiquitin specific peptidase 8
• Masse moléculaire calculée: 1118 aa, 128 kDa
• Poids moléculaire observé: 128 kDa
• Numéro d’acquisition GenBank: BC110590
• Symbole du gène: USP8
• Identification du gène (NCBI): 9101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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