Anticorps Recombinant de lapin anti-USP8

USP8 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240016D3

N° de cat : 83172-2-PBS

Synonymes

240016D3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055

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Galerie de données de validation

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  WB analysis using 83172-2-RR (same clone as 83172-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00188-1

  Sandwich ELISA standard curve of MP00188-1, Human USP8 Recombinant Matched Antibody Pair - PBS only. 83172-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27143. 83172-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  Sample test of MP00188-1

  The mean USP8 concentration was determined to be 41.15 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 47.12 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

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  IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83172-2-RR (same clone as 83172-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83172-2-RR (same clone as 83172-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00188-1

  Cytometric bead array standard curve of MP00188-1, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-1-PBS. Standard: Ag27143. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 83172-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83172-2-PBS cible USP8 dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: USP8 Protéine recombinante Ag27143
• Nom complet: ubiquitin specific peptidase 8
• Masse moléculaire calculée: 1118 aa, 128 kDa
• Poids moléculaire observé: 128 kDa
• Numéro d’acquisition GenBank: BC110590
• Symbole du gène: USP8
• Identification du gène (NCBI): 9101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

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