Anticorps Polyclonal de lapin anti-UST

UST Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27578-1-PBS

Synonymes

2OST, uronyl 2 sulfotransferase

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Galerie de données de validation

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  WB analysis using 27578-1-AP (same clone as 27578-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27578-1-AP (UST antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27578-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 27578-1-AP (same clone as 27578-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27578-1-AP (UST antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27578-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 27578-1-AP (same clone as 27578-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using UST antibody (27578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27578-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27578-1-PBS cible UST dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UST Protéine recombinante Ag25483
• Nom complet: uronyl-2-sulfotransferase
• Masse moléculaire calculée: 406 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC093668
• Symbole du gène: UST
• Identification du gène (NCBI): 10090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UST (Uronyl 2-sulfotransferase) is an enzyme that catalyzes the transfer of sulfate groups to the 2-position of uronyl residues, such as iduronyl residues in dermatan sulfate and glucuronyl residues in chondroitin sulfate (PMID: 10187838). UST is a critical regulator of melanoma cell adhesion and motility in vitro and in vivo. Abnormal expression or activity of UST has also been associated with certain diseases, such as breast pericanalicular fibroadenoma and acute retinal necrosis (PMID: 25480151).

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