Anticorps Recombinant de lapin anti-UTP18

• Phare
• Validé par KD/KO

UTP18 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241240F1

N° de cat : 84035-3-RR

Synonymes

241240F1, CGI 48, WDR50

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Galerie de données de validation

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  WB analysis of HeLa using 84035-3-RR

  WB result of UTP18 antibody (84035-3-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UTP18 transfected HeLa cells.

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  IF Staining of A431 using 84035-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using UTP18 antibody (84035-3-RR, Clone: 241240F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84035-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using UTP18 antibody (84035-3-RR, Clone: 241240F1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 84035-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84035-3-RR against Human UTP18 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84035-3-RR cible UTP18 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UTP18 Protéine recombinante Ag34662
• Nom complet: UTP18, small subunit (SSU) processome component, homolog (yeast)
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: NM_016001
• Symbole du gène: UTP18
• Identification du gène (NCBI): 51096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UTP18 (UTP18 small subunit processome component), also known as WDR50. It is expected to be located in nucleus. The protein is one part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. The calculated molecular weight of UTP18 is 62 kDa.

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