Anticorps Recombinant de lapin anti-UTY

UTY Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241626F7

N° de cat : 84332-5-RR

Synonymes

UTY1, KDM6C, KDM6AL, Histone demethylase UTY, 241626F7

---

Galerie de données de validation

• 

  WB analysis using 84332-5-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84332-5-RR (UTY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MDA-MB-231 using 84332-5-RR

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using UTY antibody (84332-5-RR, Clone: 241626F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84332-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84332-5-RR against Human UTY were performed. The affinity constant is 0.23 nM.

---

Applications testées

• Résultats positifs en WB: cellules THP-1,
• Résultats positifs en IF/ICC: cellules MDA-MB-231,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84332-5-RR cible UTY dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UTY Protéine recombinante Ag35806
• Nom complet: ubiquitously transcribed tetratricopeptide repeat gene, Y-linked
• Masse moléculaire calculée: 150kDa，1347aa
• Poids moléculaire observé: 130-160kDa
• Numéro d’acquisition GenBank: NM_007125.4
• Symbole du gène: UTY
• Identification du gène (NCBI): 7404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UTY (Ubiquitously Transcribed Tetratricopeptide Repeat Containing, Y-Linked) is a protein-coding gene located on the Y chromosome. It is the Y-linked homolog of the X-linked gene KDM6A, which encodes a histone demethylase. UTY is involved in the regulation of gene expression and chromatin structure, similar to its X-linked counterpart KDM6A (PMID: 31097364). UTY is expressed in various tissues and is predicted to be involved in transcription, translation, and nucleic acid binding, which are central to the regulation of gene expression during development, immune function, cell proliferation, and differentiation.

{{ptg:RelatedPrimaryAntibodies}}