Anticorps Polyclonal de lapin anti-Ubiquilin 1

Ubiquilin 1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 22126-1-AP

Synonymes

DA41, DSK2, hPLIC 1, PLIC 1, PLIC1, ubiquilin 1, UBQLN1, XDRP1

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Galerie de données de validation

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  WB analysis of rat brain using 22126-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 22126-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 22126-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 22126-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 22126-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 22126-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 22126-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ubiquilin 1 antibody (22126-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HEK-293 transfectées, cellules L02, tissu cardiaque de rat, tissu hépatique de rat
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22126-1-AP cible Ubiquilin 1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ubiquilin 1 Protéine recombinante Ag17530
• Nom complet: ubiquilin 1
• Masse moléculaire calculée: 589 aa, 63 kDa
• Poids moléculaire observé: 63-71 kDa
• Numéro d’acquisition GenBank: BC010066
• Symbole du gène: UBQLN1
• Identification du gène (NCBI): 29979
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.