Anticorps Recombinant de lapin anti-Ubiquilin 1

Ubiquilin 1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242092E9

N° de cat : 84649-2-PBS

Synonymes

UBQLN1, 242092E9, DA41, DSK2, hPLIC 1

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Galerie de données de validation

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  WB analysis using 84649-2-RR (same clone as 84649-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84649-2-RR (Ubiquilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84649-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84649-2-RR (same clone as 84649-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ubiquilin 1 antibody (84649-2-RR, Clone: 242092E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84649-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01462-2

  Cytometric bead array standard curve of MP01462-2, Ubiquilin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84649-2-PBS. Detection antibody: 84649-3-PBS. Standard: Ag17530. Range: 0.469-30 ng/mL.

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  Cytometric bead array standard curve of MP01462-1

  Cytometric bead array standard curve of MP01462-1, Ubiquilin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84649-2-PBS. Detection antibody: 84649-1-PBS. Standard:Ag17530. Range: 0.469-30 ng/mL.

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  Affinity and Kinetic Characterization of 84649-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84649-2-PBS against Human Ubiquilin 1 was performed. The affinity constant is 0.99 nM.

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  Affinity and Kinetic Characterization of 84649-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84649-2-RR against Human Ubiquilin 1 were performed. The affinity constant is 1.08 nM.

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Informations sur le produit

84649-2-PBS cible Ubiquilin 1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Ubiquilin 1 Protéine recombinante Ag17530
• Nom complet: ubiquilin 1
• Masse moléculaire calculée: 589 aa, 63 kDa
• Poids moléculaire observé: 63-71 kDa
• Numéro d’acquisition GenBank: BC010066
• Symbole du gène: Ubiquilin 1
• Identification du gène (NCBI): 29979
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.

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