Anticorps Recombinant de lapin anti-Ubiquilin 1

Ubiquilin 1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242092E9

N° de cat : 84649-2-RR

Synonymes

UBQLN1, hPLIC-1, hPLIC1, hPLIC 1, DSK2

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Galerie de données de validation

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  WB analysis using 84649-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84649-2-RR (Ubiquilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84649-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ubiquilin 1 antibody (84649-2-RR, Clone: 242092E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84649-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84649-2-RR against Human Ubiquilin 1 were performed. The affinity constant is 1.08 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules L02, cellules MOLT-4, cellules T-47D
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84649-2-RR cible Ubiquilin 1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Ubiquilin 1 Protéine recombinante Ag17530
• Nom complet: ubiquilin 1
• Masse moléculaire calculée: 589 aa, 63 kDa
• Poids moléculaire observé: 63-71 kDa
• Numéro d’acquisition GenBank: BC010066
• Symbole du gène: Ubiquilin 1
• Identification du gène (NCBI): 29979
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.

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