Anticorps Polyclonal de lapin anti-Unc119b

Unc119b Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 26201-1-AP

Synonymes

AA407659, AI414892, unc 119 homolog B (C. elegans), Unc119b

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Galerie de données de validation

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  WB analysis using 26201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 26201-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 26201-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 26201-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26201-1-AP (Unc119b antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules MDCK, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26201-1-AP cible Unc119b dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Unc119b Protéine recombinante Ag24599
• Nom complet: unc-119 homolog B (C. elegans)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-31 kDa
• Numéro d’acquisition GenBank: BC008617
• Symbole du gène: Unc119b
• Identification du gène (NCBI): 106840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse Unc119b is related closely to Unc119, a homolog of C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. Mouse Unc119b shares 90% sequence identity with human UNC119b protein. UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Francesc R (Verified Customer) (06-14-2022)

Great results by Western blot, with band of right size that disappears in CRISPR KO clones. In attached image, lanes 1-5 are HEK293T clones (three of them UNC119B-KO) and lanes 6-11 are hTERT-RPE1 clones (all containing the protein).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and hTERT-RPE1

Unc119b Antibody Western Blot validation (1:1000 dilution) in HEK293T and hTERT-RPE1 (Cat no:26201-1-AP)

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