Anticorps Polyclonal de lapin anti-Uromodulin

Uromodulin Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29975-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of human urine using 29975-1-AP (same clone as 29975-1-PBS)

  human urine sample were subjected to SDS PAGE followed by western blot with 29975-1-AP (Uromodulin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 29975-1-AP (same clone as 29975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 29975-1-AP (same clone as 29975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 29975-1-AP (same clone as 29975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 29975-1-AP (same clone as 29975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

29975-1-PBS cible Uromodulin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Uromodulin Protéine recombinante Ag32343
• Nom complet: uromodulin
• Masse moléculaire calculée: 640 aa, 70 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC035975
• Symbole du gène: Uromodulin
• Identification du gène (NCBI): 7369
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)

{{ptg:RelatedPrimaryAntibodies}}