Anticorps Recombinant de lapin anti-Uromodulin

Uromodulin Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242258H4

N° de cat : 85597-4-PBS

Synonymes

Tamm-Horsfall urinary glycoprotein, THP, Umod, Uromodulin, secreted form

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Galerie de données de validation

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  WB analysis using 85597-4-RR (same clone as 85597-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85597-4-RR (Umod antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85597-4-RR (Uromodulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Uromodulin antibody (85597-4-RR, Clone: 242258H4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Uromodulin antibody (85597-4-RR, Clone: 242258H4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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Informations sur le produit

85597-4-PBS cible Uromodulin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Uromodulin Protéine recombinante Eg2748
• Nom complet: Uromodulin
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: NM_009470.5
• Symbole du gène: Umod
• Identification du gène (NCBI): 22242
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin is linked to water/electrolyte balance and kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)

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