Anticorps Polyclonal de lapin anti-Uteroglobin/CC10

Uteroglobin/CC10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26909-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of rat lung using 26909-1-AP (same clone as 26909-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 26909-1-AP (Uteroglobin Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 26909-1-AP (same clone as 26909-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26909-1-AP (Uteroglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 26909-1-AP (same clone as 26909-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (26909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26909-1-PBS cible Uteroglobin/CC10 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Uteroglobin/CC10 Protéine recombinante Ag25530
• Nom complet: secretoglobin, family 1A, member 1 (uteroglobin)
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 9 kDa
• Numéro d’acquisition GenBank: BC004481
• Symbole du gène: Uteroglobin
• Identification du gène (NCBI): 7356
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

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