Anticorps Polyclonal de lapin anti-VAMP1

VAMP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13115-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human brain using 13115-1-AP (same clone as 13115-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13115-1-AP (VAMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 13115-1-AP (same clone as 13115-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 13115-1-AP (VAMP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13115-1-PBS cible VAMP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAMP1 Protéine recombinante Ag3787
• Nom complet: vesicle-associated membrane protein 1 (synaptobrevin 1)
• Masse moléculaire calculée: 117 aa, 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC023286
• Symbole du gène: VAMP1
• Identification du gène (NCBI): 6843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VAMP1, also named as synaptobrevin 1, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP1 is a part of the SNARE (soluble NSF-attachment protein receptor) complex. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP1 is involved in synaptic vesicle exocytosis, a fundamental step in neurotransmitter release.

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