Anticorps Polyclonal de lapin anti-VAMP2

VAMP2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Goat / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 30001-0-AP

Synonymes

SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Galerie de données de validation

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• ELISA: See 3 publications below

Informations sur le produit

30001-0-AP cible VAMP2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Goat / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: NM_014232
• Symbole du gène: VAMP2
• Identification du gène (NCBI): 6844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family of proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2 consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Publications

Species	Application	Title
human	ELISA	J Cell Biochem; Un-nicked BoNT/B activity in human SHSY-5Y neuronal cells.; Authors - Shi Xuerong X
human	ELISA	J Cell Biochem; TCEP treatment reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells.; Authors - Shi Xuerong X
human	ELISA	J Cell Biochem; The biological activity of ubiquitinated BoNT/B light chain in vitro and in human SHSY-5Y neuronal cells.; Authors - Shi Xuerong X
human	WB	Mol Neurobiol; L116 Deletion in CSPα Promotes α-Synuclein Aggregation and Neurodegeneration; Authors - Tao Guo

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