Anticorps Monoclonal anti-VAMP2

VAMP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G7E8

Avis (1)

N° de cat : 67822-1-Ig

Synonymes

FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Galerie de données de validation

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  WB analysis using 67822-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67822-1-Ig (VAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67822-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67822-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-87 MG using 67822-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using VAMP2 antibody (67822-1-Ig, Clone: 1G7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of SH-SY5Y using 67822-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using VAMP2 antibody (67822-1-Ig, Clone: 1G7E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MG U-87, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67822-1-Ig cible VAMP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VAMP2 Protéine recombinante Ag17908
• Nom complet: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC002737
• Symbole du gène: VAMP2
• Identification du gène (NCBI): 6844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Michela (Verified Customer) (06-20-2024)

The immunofluorescence was carried out in primary hippocampal neurons with an antibody dilution of 1:500. The antibody appears to be very specific and with minimal background staining.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary Hipppocampal Neurons

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