Anticorps Monoclonal anti-VAMP3/Cellubrevin

VAMP3/Cellubrevin Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D10D10

N° de cat : 66488-1-PBS

Synonymes

VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB

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Galerie de données de validation

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  WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

  Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66488-1-Ig (same clone as 66488-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 66488-1-Ig (same clone as 66488-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using VAMP3/Cellubrevin antibody (66488-1-Ig, Clone: 1D10D10 ) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66488-1-PBS cible VAMP3/Cellubrevin dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VAMP3/Cellubrevin Protéine recombinante Ag1156
• Nom complet: vesicle-associated membrane protein 3 (cellubrevin)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC005941
• Symbole du gène: VAMP3
• Identification du gène (NCBI): 9341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VAMP3, also named cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

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