Anticorps Polyclonal de lapin anti-VAMP5

VAMP5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 11822-1-AP

Synonymes

Myobrevin, VAMP 5, VAMP5

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Galerie de données de validation

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  WB analysis of human lung using 11822-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11822-1-AP (VAMP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11822-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11822-1-AP (VAMP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood leukocyte using 11822-1-AP

  Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 11822-1-AP (VAMP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11822-1-AP (VAMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11822-1-AP (VAMP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire humain, cellules leucocytaires de sang périphérique humain, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

11822-1-AP cible VAMP5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAMP5 Protéine recombinante Ag2390
• Nom complet: vesicle-associated membrane protein 5 (myobrevin)
• Masse moléculaire calculée: 12.8 kDa
• Poids moléculaire observé: 13-15 kDa
• Numéro d’acquisition GenBank: BC017891
• Symbole du gène: VAMP5
• Identification du gène (NCBI): 10791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP5, also known as myobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP5 may participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking.

Publications

Species	Application	Title
human	WB,IHC	Front Immunol; Immune Gene Signatures and Immunotypes in Immune Microenvironment Are Associated With Glioma Prognose.; Authors - Xiang-Xu Wang
human	WB	Front Mol Neurosci; Construction and validation of a risk model based on the key SNARE proteins to predict the prognosis and immune microenvironment of gliomas; Authors - Luxin Yin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (06-19-2020)

This antibody labels a ~ 25 kD band, but not a 15 kD band, in 293T cell lysate - not sure is this a nonspecific band or a special version of this protein in 293T cells.

• Applications: Western Blot