Anticorps Polyclonal de lapin anti-VAMP7/TI-VAMP

• Phare
• Validé par KD/KO

VAMP7/TI-VAMP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22268-1-PBS

Synonymes

VAMP7, TI VAMP, Tetanus-insensitive VAMP, Tetanus insensitive VAMP, Synaptobrevin-like protein 1

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Galerie de données de validation

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  WB analysis using 22268-1-AP (same clone as 22268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22268-1-AP (same clone as 22268-1-PBS)

  IP result of anti-VAMP7/TI-VAMP (IP:22268-1-AP, 4ug; Detection:22268-1-AP 1:1000) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAMP7/TI-VAMP antibody (22268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22268-1-PBS cible VAMP7/TI-VAMP dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: vesicle-associated membrane protein 7
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: NM_005638
• Symbole du gène: VAMP7
• Identification du gène (NCBI): 6845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VAMP7, also named SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during the transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is necessary for calcium-regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.

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