Anticorps Polyclonal de lapin anti-VANGL2

• Phare
• Validé par KD/KO

VANGL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21492-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 21492-1-AP (same clone as 21492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  WB analysis using 21492-1-AP (same clone as 21492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 21492-1-AP (same clone as 21492-1-PBS)

  IP result of anti-VANGL2 (IP:21492-1-AP, 4ug; Detection:21492-1-AP 1:500) with HepG2 cells lysate 1880 ug. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 21492-1-AP (same clone as 21492-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 21492-1-AP (same clone as 21492-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21492-1-AP (same clone as 21492-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 21492-1-AP (VANGL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21492-1-PBS cible VANGL2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VANGL2 Protéine recombinante Ag15833
• Nom complet: vang-like 2 (van gogh, Drosophila)
• Masse moléculaire calculée: 521 aa, 60 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC103920
• Symbole du gène: VANGL2
• Identification du gène (NCBI): 57216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.

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