Anticorps Polyclonal de lapin anti-VAPB

• Phare
• Validé par KD/KO

VAPB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (1)

N° de cat : 14477-1-AP

Synonymes

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB

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Galerie de données de validation

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  WB analysis of HepG2 using 14477-1-AP

  WB result of VAPB antibody (14477-1-AP, 1:5000) with si-control and si-VAPB transfected HepG2 cells.

• 

  WB analysis using 14477-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14477-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14477-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14477-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14477-1-AP

  IP Result of anti-VAPB (IP:14477-1-AP, 3ug; Detection:14477-1-AP 1:800) with HeLa cells lysate 1840ug.

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14477-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAPB antibody (14477-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 14477-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VAPB antibody (14477-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 14477-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug VAPB antibody (14477-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du pancréas humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14477-1-AP cible VAPB dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAPB Protéine recombinante Ag5857
• Nom complet: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC001712
• Symbole du gène: VAPB
• Identification du gène (NCBI): 9217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Publications

Species	Application	Title
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
monkey	WB	Mol Cell; MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.; Authors - Christophe A C Freyre
human	WB	J Clin Invest; SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.; Authors - Museer A Lone
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (09-22-2020)

The antibody have predicted band on western blot.

• Applications: Western Blot
• Cell Tissue Type: Mouse heart

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