Anticorps Monoclonal anti-VAPB

• Phare
• Validé par KD/KO

VAPB Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

CloneNo.

4F6A6

N° de cat : 66191-1-Ig

Synonymes

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB

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Galerie de données de validation

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  WB analysis of HepG2 using 66191-1-Ig

  WB result of VAPB antibody (66191-1-Ig, 1:5000) with si-control and si-VAPB transfected HepG2 cells.

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  WB analysis using 66191-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66191-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 66191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66191-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPB antibody (66191-1-Ig, Clone: 4F6A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66191-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66191-1-Ig (VAPB antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

66191-1-Ig cible VAPB dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, levure, singe
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VAPB Protéine recombinante Ag6313
• Nom complet: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC001712
• Symbole du gène: VAPB
• Identification du gène (NCBI): 9217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Publications

Species	Application	Title
human	WB	EMBO J; CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.; Authors - Thaddaeus Mutugi Nthiga
monkey	WB, CoIP,IF	Curr Biol; The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.; Authors - Yan G Zhao ; KD Validated
human	WB	J Cell Biol; Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.; Authors - Nan Liu
human	WB	J Biol Chem; Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.; Authors - Christina James
human	CoIP	J Biochem; MITOL regulates phosphatidic acid-binding activity of RMDN3/PTPIP51.; Authors - Naoki Ito
human,monkey	WB,IF	Contact (Thousand Oaks); Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells.; Authors - Alexa Bishop