Anticorps Monoclonal anti-VAPB

• Phare
• Validé par KD/KO

VAPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4F6A6

N° de cat : 66191-1-PBS

Synonymes

4F6A6, ALS8, UNQ484/PRO983, VAMP associated protein B/C, VAMP B

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Galerie de données de validation

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  WB analysis of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

  WB result of VAPB antibody (66191-1-Ig, 1:5000) with si-control and si-VAPB transfected HepG2 cells. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66191-1-Ig (VAPB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPB antibody (66191-1-Ig, Clone: 4F6A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66191-1-Ig (VAPB antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66191-1-PBS cible VAPB dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VAPB Protéine recombinante Ag6313
• Nom complet: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC001712
• Symbole du gène: VAPB
• Identification du gène (NCBI): 9217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

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