Anticorps Polyclonal de lapin anti-VASH1

• Phare
• Validé par KD/KO

VASH1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12730-1-AP

Synonymes

Tyrosine carboxypeptidase 1, Tubulinyl-Tyr carboxypeptidase 1, Tubulin carboxypeptidase 1, TTCP 1, KIAA1036

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Galerie de données de validation

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  WB analysis of human brain using 12730-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12730-1-AP (VASH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12730-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12730-1-AP (VASH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12730-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12730-1-AP (VASH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 12730-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using VASH1 antibody (12730-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa, cellules MCF-7
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12730-1-AP cible VASH1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VASH1 Protéine recombinante Ag3435
• Nom complet: vasohibin 1
• Masse moléculaire calculée: 365 aa, 41 kDa
• Poids moléculaire observé: 36 and 41 kDa
• Numéro d’acquisition GenBank: BC009031
• Symbole du gène: VASH1
• Identification du gène (NCBI): 22846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VASH1, also named as vasohibin 1, is a angiogenesis inhibitor induced by the stimulation with VEGF. High expression of VASH1 has been researched in some tumors as a result of VEGF induction. It has been suggested that VASH-1 is clinically significant in bladder cancer, colorectal cancer and NSCLC. VASH-1 may be a potential marker of gastric cancer and a target for vascular targeting therapy. 12730-1-AP detects 41 kDa form secreted protein and 36 kDa form protein accumulated within the cells or pericellular milieu in SDS-PAGE.(PMID:19057892, 29042936, 29552154, 26666821)

Publications

Species	Application	Title
zebrafish	WB	Development; Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk.; Authors - Marta Bastos de Oliveira ; KO Validated
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated
mouse	IF	Neuron; MicroRNA mechanisms instructing Purkinje cell specification; Authors - Norjin Zolboot

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