Anticorps Monoclonal anti-VASH2

VASH2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A11D10

N° de cat : 67753-1-PBS

Synonymes

Vasohibin-like protein, Vasohibin-2, VASHL, Tubulinyl-Tyr carboxypeptidase 2, EC:3.4.17.17

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Galerie de données de validation

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  WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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  WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67753-1-Ig (same clone as 67753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67753-1-PBS cible VASH2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VASH2 Protéine recombinante Ag29985
• Nom complet: vasohibin 2
• Masse moléculaire calculée: 355 aa, 40 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC028194
• Symbole du gène: VASH2
• Identification du gène (NCBI): 79805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)

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