Anticorps Polyclonal de lapin anti-VASP

• Phare
• Validé par KD/KO

VASP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 13472-1-AP

Synonymes

VASP

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Galerie de données de validation

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  WB analysis using 13472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13472-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13472-1-AP

  IP result of anti-VASP(IP:13472-1-AP, 4ug; Detection:13472-1-AP 1:10000) with HEK-293 cells lysate 920 ug.

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  IHC staining of human testis using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin cancer using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin cancer using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 13472-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VASP antibody (13472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 13472-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug VASP antibody (13472-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules HEK-293, cellules HeLa, tissu splénique de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu de cancer de la peau humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

13472-1-AP cible VASP dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VASP Protéine recombinante Ag4266
• Nom complet: vasodilator-stimulated phosphoprotein
• Masse moléculaire calculée: 380 aa, 40 kDa
• Poids moléculaire observé: 46 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC038224
• Symbole du gène: VASP
• Identification du gène (NCBI): 7408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.

Publications

Species	Application	Title
human	IF	Nat Commun; Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.; Authors - Thibault Courtheoux
human	WB	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv
human	WB, IF, IHC	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li ; KD Validated
human	WB	Front Oncol; High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.; Authors - Jun Wang
human	WB	Oncol Rep; TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway.; Authors - Wen Liu ; KD Validated
human	WB	Pathol Res Pract; Silencing lncRNA SNHG6 suppresses proliferation and invasion of breast cancer cells through miR-26a/VASP axis.; Authors - Kai Li