Anticorps Monoclonal anti-VASP

VASP Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B9A11

N° de cat : 68431-1-PBS

Synonymes

1B9A11, Vasodilator-stimulated phosphoprotein

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Galerie de données de validation

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  WB analysis using 68431-1-Ig (same clone as 68431-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68431-1-Ig (VASP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68431-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51452-1

  Cytometric bead array sample test of MP51452-1, VASP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68431-1-PBS. Detection antibody: 68431-2-PBS.

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  Cytometric bead array standard curve of MP51452-1

  Cytometric bead array standard curve of MP51452-1, VASP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68431-1-PBS. Detection antibody: 68431-2-PBS. Standard:Ag17679. Range: 1.563-200 ng/mL.

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Informations sur le produit

68431-1-PBS cible VASP dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VASP Protéine recombinante Ag17679
• Nom complet: vasodilator-stimulated phosphoprotein
• Masse moléculaire calculée: 380 aa, 40 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC038224
• Symbole du gène: VASP
• Identification du gène (NCBI): 7408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.

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