Anticorps Polyclonal de lapin anti-VAV1

• Phare
• Validé par KD/KO

VAV1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 16364-1-AP

Synonymes

Proto oncogene vav, Proto-oncogene vav, VAV

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Galerie de données de validation

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  WB analysis using 16364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16364-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16364-1-AP

  IP result of anti-VAV1 (IP:16364-1-AP, 4ug; Detection:16364-1-AP 1:4000) with K-562 cells lysate 1800 ug.

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules HL-60, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque humain, tissu cérébral humain, tissu d'amygdalite humain, tissu de lymphome humain, tissu hépatique humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

16364-1-AP cible VAV1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris, Gecko
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAV1 Protéine recombinante Ag9049
• Nom complet: vav 1 guanine nucleotide exchange factor
• Masse moléculaire calculée: 845 aa, 98 kDa
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: BC013361
• Symbole du gène: VAV1
• Identification du gène (NCBI): 7409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vav proteins mainly act as enzymes that catalyze the activation step of Rho subfamily GTPases during cell signaling. There are three family members:VAV1, VAV2 and VAV3. Vav1 is specifically expressed in the hematopoietic system, whereas Vav2 and Vav3 are more ubiquitously expressed (PMID:14607270). Vav1 is physiologically active as a GDP/GTP nucleotide exchange factor (GEF) in the hematopoietic system (PMID:31654719).

Publications

Species	Application	Title
gecko	WB,IF	J Biol Chem; Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.; Authors - Nan Du ; KD Validated
human	WB,IHC	Biochem Biophys Res Commun; Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis.; Authors - Xiaolei Zhu
human	WB	Oncotarget; Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.; Authors - Wei-Zhen Xue
rat	WB,IF	Clin Sci (Lond); Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.; Authors - Chang-Yien Chan ; KD Validated
human	WB	Cancer Cell Int; STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment; Authors - Zhangya Pu
rat	IF	Neural Regen Res; Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation; Authors - Jing Qiu

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