Anticorps Polyclonal de lapin anti-VAV3

VAV3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30291-1-PBS

Synonymes

Guanine nucleotide exchange factor VAV3, VAV 3

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Galerie de données de validation

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  WB analysis using 30291-1-AP (same clone as 30291-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30291-1-AP (VAV3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 30291-1-AP (same clone as 30291-1-PBS)

  IP result of anti-VAV3 (IP:30291-1-AP, 4ug; Detection:30291-1-AP 1:8000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 30291-1-AP (same clone as 30291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30291-1-AP (VAV3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 30291-1-AP (same clone as 30291-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV3 antibody (30291-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 30291-1-AP (same clone as 30291-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using VAV3 antibody (30291-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 30291-1-AP (same clone as 30291-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human VAV3 (30291-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30291-1-PBS cible VAV3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAV3 Protéine recombinante Ag33022
• Nom complet: vav 3 guanine nucleotide exchange factor
• Masse moléculaire calculée: 98kd
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: NM_006113
• Symbole du gène: VAV3
• Identification du gène (NCBI): 10451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vav3, a member of Dbl family which is also the activator of GTP kinase of Rho family, can activate the expression of RhoA, Racl and Cdc42 (PMID:28285969). Vav3 is associated with tumor growth, apoptosis, invasion, metastasis and angiogenesis (PMID:28285969). Vav3 is a multiple function protein with both signaling molecule and coactivator activities. Vav3 oncogene is overexpressed in androgen-independent prostate cancer cells relative to their androgen-dependent counterparts and contribute to development of hormone refractory prostate cancer (PMID:21455584).

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