Anticorps Polyclonal de lapin anti-VCAM-1/CD106

VCAM-1/CD106 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 30958-1-AP

Synonymes

CD106, VCAM-1, Vcam1, Vcam 1

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Galerie de données de validation

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  WB analysis using 30958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30958-1-AP (VCAM-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 30958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 30958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique de rat, cellules C2C12, tissu splénique de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

30958-1-AP cible VCAM-1/CD106 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: vascular cell adhesion molecule 1
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: CAA47989.1
• Symbole du gène: VCAM-1
• Identification du gène (NCBI): 22329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publications

Species	Application	Title
mouse	IHC	Microvasc Res; Characterization of microcirculatory endothelial functions in a D-Galactose-induced aging model; Authors - Zhuo Li
mouse	IF	Redox Biol; Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation; Authors - Hui He

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