Anticorps Monoclonal anti-VCAM-1/CD106

VCAM-1/CD106 Monoclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(37)

CloneNo.

1B12F12

N° de cat : 66294-1-Ig

Synonymes

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

---

Galerie de données de validation

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1/CD106 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1/CD106 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66294-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1/CD106 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66294-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

66294-1-Ig cible VCAM-1/CD106 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VCAM-1/CD106 Protéine recombinante Ag24697
• Nom complet: vascular cell adhesion molecule 1
• Masse moléculaire calculée: 739 aa, 81 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC017276
• Symbole du gène: VCAM-1
• Identification du gène (NCBI): 7412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publications

Species	Application	Title
	IF	Redox Biol; Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.; Authors - Zhuqing Li
human		J Hazard Mater; Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells.; Authors - Xuting Liu
human		Int J Biol Macromol; Novel soybean polypeptide dglycin alleviates atherosclerosis in apolipoprotein E-deficient mice; Authors - Han Zhao
human		Life Sci; PMID: 29452166; Authors - Qiangbing Yang
human		Int J Mol Sci; Hypoxic Culture Maintains Cell Growth of the Primary Human Valve Interstitial Cells with Stemness.; Authors - Kaho Kanno
		J Nanobiotechnology; Macrophage-cancer hybrid membrane-coated nanoparticles for targeting lung metastasis in breast cancer therapy.; Authors - Chunai Gong

{{ptg:RelatedPrimaryAntibodies}}