Anticorps Monoclonal anti-VCAM-1/CD106

VCAM-1/CD106 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B12F12

N° de cat : 66294-1-PBS

Synonymes

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

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Galerie de données de validation

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  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1/CD106 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1/CD106 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1/CD106 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66294-1-PBS cible VCAM-1/CD106 dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VCAM-1/CD106 Protéine recombinante Ag24697
• Nom complet: vascular cell adhesion molecule 1
• Masse moléculaire calculée: 739 aa, 81 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC017276
• Symbole du gène: VCAM-1
• Identification du gène (NCBI): 7412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

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