Anticorps Recombinant de lapin anti-VCAM-1/CD106

VCAM-1/CD106 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241283G8

N° de cat : 98079-1-PBS

Synonymes

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

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Galerie de données de validation

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  FC experiment of HUVEC using 98079-1-RR (same clone as 98079-1-PBS)

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8)(red), or 0.25 ug Isotype Control (blue), or 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(black). Cells were not fixed. This data was developed using the same antibody clone with 98079-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 98079-1-RR (same clone as 98079-1-PBS)

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98079-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 98079-1-RR (same clone as 98079-1-PBS)

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98079-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98079-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98079-1-RR against Human VCAM-1/CD106 were performed. The affinity constant is 1.71 nM.

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Informations sur le produit

98079-1-PBS cible VCAM-1/CD106 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: vascular cell adhesion molecule 1
• Masse moléculaire calculée: 81 kDa
• Numéro d’acquisition GenBank: NM_001078.4
• Symbole du gène: VCAM-1
• Identification du gène (NCBI): 7412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

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