Anticorps Monoclonal anti-VCP

• Phare
• Validé par KD/KO

VCP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ChIP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2A4B10

N° de cat : 60316-1-Ig

Synonymes

15S Mg(2+) ATPase p97 subunit, 15S Mg(2+)-ATPase p97 subunit, 2A4B10, EC:3.6.4.6, HEL-220

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Galerie de données de validation

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  WB analysis of U2OS using 60316-1-Ig

  U2OS (WT and VCP KD) lysates prepared with RIPA buffer, 10μg protein loaded. 60316-1-Ig incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 60316-1-Ig

  WB result of VCP antibody (60316-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells.

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  WB analysis of RAW 264.7 using 60316-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 60316-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60316-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (60316-1-Ig, Clone: 2A4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of SH-SY5Y using 60316-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60316-1-Ig(VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HL-60 using 60316-1-Ig

  1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (60316-1-Ig, Clone:2A4B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris, tissu de cancer de l'estomac humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain, tissu de côlon de souris, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules SH-SY5Y
• Résultats positifs en FC (Intra): cellules HL-60,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

60316-1-Ig cible VCP dans les applications de WB, IHC, IF/ICC, FC (Intra), ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VCP Protéine recombinante Ag1002
• Nom complet: valosin-containing protein
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC007562
• Symbole du gène: VCP
• Identification du gène (NCBI): 7415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Publications

Species	Application	Title
human	WB,IF	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič ; KD Validated
human	WB	Acta Pharmacol Sin; Gossypol, a novel modulator of VCP, induces autophagic degradation of mutant huntingtin by promoting the formation of VCP/p97-LC3-mHTT complex.; Authors - Xiao-Jing Li
human	WB,ChIP	Biochim Biophys Acta Gene Regul Mech; GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1; Authors - Luciana L Almada
human	WB,IF	Research (Wash D C); SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage; Authors - Fengli Wang
human	IF	Cell; RNA damage compartmentalization by DHX9 stress granules; Authors - Yilong Zhou
human	WB	Sci Adv; p37 regulates VCP/p97 shuttling and functions in the nucleus and cytosol; Authors - Lidia Wrobel ; KD Validated

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