Anticorps Polyclonal de lapin anti-VDAC1/2/3

VDAC1/2/3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 11663-1-AP

Synonymes

hVDAC2, VDAC 2, VDAC2

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Galerie de données de validation

• 

  WB analysis using 11663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11663-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu prostatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

11663-1-AP cible VDAC1/2/3 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VDAC1/2/3 Protéine recombinante Ag2266
• Nom complet: voltage-dependent anion channel 2
• Masse moléculaire calculée: 294 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000165
• Symbole du gène: VDAC2
• Identification du gène (NCBI): 7417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and expecially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue.(PMID: 22020053)

Publications

Species	Application	Title
mouse	WB	Nat Commun; Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.; Authors - Shintaro Mise
mouse	IP	Autophagy; MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals.; Authors - Jia Yuan
human,mouse	WB,IF	Cell Death Differ; SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy.; Authors - Ying Zhang
mouse	WB	Proc Natl Acad Sci U S A; SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.; Authors - Haruhiko Miyata
rat	WB,CoIP	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang
human	WB	EMBO Mol Med; Conformational change of adenine nucleotide translocase-1 mediates cisplatin resistance induced by EBV-LMP1.; Authors - Lin Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jun (Verified Customer) (06-12-2022)

Works very well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

VDAC1/2/3 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11663-1-AP)

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