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Anticorps Polyclonal de lapin anti-VDAC2

VDAC2 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 55261-1-AP

Synonymes

hVDAC2, VDAC 2, VDAC2

Galerie de données de validation

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  • Western Blot (WB) analysis of HepG2 cells using VDAC2 Polyclonal antibody (55261-1-AP)

    WB analysis of HepG2 using 55261-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    WB analysis of mouse brain using 55261-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using VDAC2 Polyclonal antibody (55261-1-AP)

    WB analysis of HuH-7 using 55261-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human heart using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human heart using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

Applications testées

Résultats positifs en WBcellules HepG2, cellules HuH-7, tissu cérébral de souris
Résultats positifs en IHCtissu rénal humain, tissu cardiaque humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

55261-1-AP cible VDAC2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet voltage-dependent anion channel 2
Masse moléculaire calculée 33 kDa
Poids moléculaire observé 31-33 kDa
Numéro d’acquisition GenBankNM_003375
Symbole du gène VDAC2
Identification du gène (NCBI) 7417
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC2.

Protocole

Product Specific Protocols
WB protocol for VDAC2 antibody 55261-1-APDownload protocol
IHC protocol for VDAC2 antibody 55261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Physiol Biochem

Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress.

Authors - Meiying Yang
View Article