Anticorps Monoclonal anti-VDAC2

VDAC2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1D8C11

N° de cat : 66388-1-Ig

Synonymes

1D8C11, FLJ23841, hVDAC2, VDAC 2, VDAC-2

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Galerie de données de validation

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  WB analysis of HEK-293 using 66388-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66388-1-Ig (VDAC2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66388-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66388-1-Ig (VDAC2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules ROS1728
• Résultats positifs en IHC: tissu cardiaque humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

66388-1-Ig cible VDAC2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: voltage-dependent anion channel 2
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: NM_003375
• Symbole du gène: VDAC2
• Identification du gène (NCBI): 7417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Publications

Species	Application	Title
human	WB	Cell Rep; Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia; Authors - Yan Tang
human	WB,IHC	Phytomedicine; Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma.; Authors - Fuhao Huang
human	WB	Drug Dev Res; Xanthatin inhibits non-small cell lung cancer proliferation by breaking the redox balance.; Authors - Yanbo Xie
human	WB	Cancer Discov; BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers; Authors - Guang Lei

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