Anticorps Recombinant de lapin anti-VDAC2

VDAC2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241569G11

N° de cat : 84225-2-PBS

Synonymes

241569G11, FLJ23841, hVDAC2, VDAC 2, VDAC-2

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Galerie de données de validation

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  WB analysis using 84225-2-RR (same clone as 84225-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 84225-2-RR (same clone as 84225-2-PBS)

  IP result of anti-VDAC2 (IP:84225-2-RR, 4ug; Detection:84225-2-RR 1:2000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84225-2-RR (same clone as 84225-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-2-RR (VDAC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84225-2-RR (same clone as 84225-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-2-RR (VDAC2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VDAC2 antibody (84225-2-RR, Clone: 241569G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01162-1

  Cytometric bead array standard curve of MP01162-1, VDAC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84225-2-PBS. Detection antibody: 84225-1-PBS. Standard: Ag2266. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 84225-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84225-2-RR against Human VDAC2 were performed. The affinity constant is 0.48 nM.

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Informations sur le produit

84225-2-PBS cible VDAC2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VDAC2 Protéine recombinante Ag2266
• Nom complet: voltage-dependent anion channel 2
• Masse moléculaire calculée: 294 aa, 32 kDa
• Poids moléculaire observé: 31-33 kDa
• Numéro d’acquisition GenBank: BC000165
• Symbole du gène: VDAC2
• Identification du gène (NCBI): 7417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)

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