Anticorps Recombinant de lapin anti-VDAC2

VDAC2 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241569C3

N° de cat : 84225-3-PBS

Synonymes

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569C3

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Galerie de données de validation

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  IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VDAC2 antibody (84225-3-RR, Clone: 241569C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-3-RR, Clone:241569C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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Informations sur le produit

84225-3-PBS cible VDAC2 dans les applications de IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VDAC2 Protéine recombinante Ag2266
• Nom complet: voltage-dependent anion channel 2
• Masse moléculaire calculée: 294 aa, 32 kDa
• Numéro d’acquisition GenBank: BC000165
• Symbole du gène: VDAC2
• Identification du gène (NCBI): 7417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)

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