Anticorps Recombinant de lapin anti-VE-cadherin/CD144

VE-cadherin/CD144 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240755E10

N° de cat : 83766-5-RR

Synonymes

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Galerie de données de validation

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  WB analysis using 83766-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83766-5-RR (VE-cadherin/CD144 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83766-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83766-5-RR against Human VE-cadherin were performed. The affinity constant is 51.2 nM.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

83766-5-RR cible VE-cadherin/CD144 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cadherin 5, type 2 (vascular endothelium)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001795.5
• Symbole du gène: VE-cadherin
• Identification du gène (NCBI): 1003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Publications

Species	Application	Title
rat	WB	Neurochem Int; Kaempferol promotes angiogenesis through HIF-1α/VEGF-A/Notch1 pathway in ischemic stroke rats; Authors - Sen Zhang

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