Anticorps Monoclonal anti-VE-cadherin/CD144

VE-cadherin/CD144 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(3)

CloneNo.

4F9B10

N° de cat : CL594-66804

Synonymes

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Galerie de données de validation

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  IF Staining of human breast cancer using CL594-66804

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 VE-cadherin antibody (CL594-66804, Clone: 4F9B10 ) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL594-66804

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 VE-cadherin antibody (CL594-66804, Clone: 4F9B10 ) at dilution of 1:50.

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du sein humain, tissu d'amygdalite humain

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

CL594-66804 cible VE-cadherin/CD144 dans les applications de WB, IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VE-cadherin/CD144 Protéine recombinante Ag27501
• Nom complet: cadherin 5, type 2 (vascular endothelium)
• Masse moléculaire calculée: 88 kDa
• Numéro d’acquisition GenBank: NM_001795
• Symbole du gène: VE-cadherin
• Identification du gène (NCBI): 1003
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). E-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).

Publications

Species	Application	Title
human	WB,IF	Biochem Biophys Res Commun; Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage; Authors - Jun He
human	IF	Biomacromolecules; Interpenetrating Polymer Network HA/Alg-RGD Hydrogel: An Equilibrium of Macroscopic Stability and Microscopic Adaptability for 3D Cell Growth and Vascularization; Authors - Yuanshan Liu
human	IF	Biochem Biophys Res Commun; Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission; Authors - Xi Mei

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