Anticorps Polyclonal de lapin anti-VE-cadherin/CD144

VE-cadherin/CD144 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 32332-1-AP

Synonymes

VE-cadherin, 7B4, cadherin 5, Cd144, Cdh5

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Galerie de données de validation

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  WB analysis using 32332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32332-1-AP (VE-cadherin/CD144 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of bEnd.3 using 32332-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed bEnd.3 cells using VE-cadherin/CD144 antibody (32332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules bEnd.3, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IF/ICC: cellules bEnd.3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32332-1-AP cible VE-cadherin/CD144 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VE-cadherin/CD144 Protéine recombinante Eg0921
• Nom complet: cadherin 5
• Masse moléculaire calculée: 88kDa
• Poids moléculaire observé: 130 kDa, 100 kDa
• Numéro d’acquisition GenBank: NM_009868.4
• Symbole du gène: Cdh5
• Identification du gène (NCBI): 12562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

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