Anticorps Recombinant de lapin anti-VE-cadherin/CD144

VE-cadherin/CD144 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240755D5

N° de cat : 83766-6-RR

Synonymes

VE-cadherin, 240755D5, 7B4, 7B4 antigen, Cadherin 5

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Galerie de données de validation

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  WB analysis using 83766-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83766-6-RR (VE-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83766-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-6-RR (VE-cadherin/CD144 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83766-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-6-RR (VE-cadherin/CD144 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 83766-6-RR

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin/CD144 antibody (83766-6-RR, Clone: 240755D5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HUVEC using 83766-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin/CD144 antibody (83766-6-RR, Clone: 240755D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83766-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83766-6-RR against Human VE-cadherin/CD144 were performed. The affinity constant is 0.633 nM.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

83766-6-RR cible VE-cadherin/CD144 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VE-cadherin/CD144 Protéine recombinante Eg1200
• Nom complet: cadherin 5, type 2 (vascular endothelium)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 90-100 kDa, 130-140 kDa
• Numéro d’acquisition GenBank: NM_001795.5
• Symbole du gène: VE-cadherin
• Identification du gène (NCBI): 1003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Publications

Species	Application	Title
human	WB,IF	J Cell Mol Med; Single-Cell Transcriptomic Reveals the Involvement of Cell-Cell Junctions in the Early Development of Hypertrophic Cardiomyopathy; Authors - Dingchen Wang

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