Anticorps Recombinant de lapin anti-VE-cadherin/CD144

VE-cadherin/CD144 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241953A12

N° de cat : 84529-4-RR

Synonymes

VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5

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Galerie de données de validation

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  WB analysis using 84529-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84529-4-RR (VE-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84529-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84529-4-RR against Mouse VE-cadherin/CD144 were performed. The affinity constant is 51.6 nM.

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Applications testées

• Résultats positifs en WB: cellules bEnd.3,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84529-4-RR cible VE-cadherin/CD144 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cadherin 5
• Masse moléculaire calculée: 88kDa
• Poids moléculaire observé: 125-130 kDa, 90-100 kDa
• Numéro d’acquisition GenBank: NM_009868.4
• Symbole du gène: Cdh5
• Identification du gène (NCBI): 12562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

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