Anticorps Recombinant de lapin anti-VE-cadherin/CD144

VE-cadherin/CD144 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240755B2

N° de cat : 98071-1-RR

Synonymes

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Galerie de données de validation

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  FC experiment of HUVEC using 98071-1-RR

  1x10^6 HUVEC cells were stained with 0.25 ug Anti-Human VE-cadherin/CD144 Rabbit Recombinant Antibody (98071-1-RR, Clone:240755B2) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98071-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98071-1-RR against Human VE-cadherin/CD144 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en cytométrie: cellules HUVEC,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98071-1-RR cible VE-cadherin/CD144 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cadherin 5, type 2 (vascular endothelium)
• Masse moléculaire calculée: 88 kDa
• Numéro d’acquisition GenBank: NM_001795.5
• Symbole du gène: VE-cadherin
• Identification du gène (NCBI): 1003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

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